One question that excites many researchers in synthetic biology is that – can we build a cell from scratch? With the development in the relevant areas of research, there has been an increasing drive attempting to create models that mimic a living biological cell. Some of the critical questions behind the drive are – Are we able to produce molecules efficiently as a cell does? How can we effectively understand what happens in biochemical pathways? Moreover, can we use the knowledge of the deviations from the pathways that cause diseases to mediate them? Indeed, the attempt to build a system that could answer these questions, although it may sound simple on the scale of a single cell, has been a great challenge.